Histological assessment of cellular changes in gingival epithelium in ante-mortem and post-mortem specimens


G Pradeep
K Uma
P Sharada
Nilima Prakash


Background: Death is an irreversible cessation of circulation, respiration and cerebral function. A number of continuous changes occur in the period after death, and these vary according to the duration of time interval and circumstances of death. Since the rate of post-mortem changes varies between different individuals and there exists a range of normal values during life, extrapolation of the time since death will always reveal an interval, not an exact time point at which death occurred. Objectives: This study was conducted to demonstrate the features of decomposition at the cellular level in post-mortem gingival tissues at different time intervals after death and observe the cellular changes in the unfixed ante-mortem gingival tissue at regular time intervals. Materials and Methods: Thirty post-mortem and 30 ante-mortem gingival samples were seen microscopically to observe the cytoplasmic and nuclear degenerative changes. The proportion of cells exhibiting nuclear and cytoplasmic alterations in post-mortem gingival samples, during different time intervals, were compared using Chi square test. Results and Conclusion: There was a significant association between the time intervals and degenerative changes. The initiation of the decomposition process at the cellular level begins within 10 hours after death and the other clinical features of decomposition occur subsequently.


How to Cite
G Pradeep, K Uma, P Sharada, & Nilima Prakash. (2009). Histological assessment of cellular changes in gingival epithelium in ante-mortem and post-mortem specimens. Journal of Forensic Dental Sciences, 1(2), 61–65. https://doi.org/10.4103/0974-2948.60375


  1. Walter JB,Talbot IC. Walter and Israel General Pathology. 7th ed. Churchill Livingstone; 1996.
  2. Mohan H. Text book of Pathology. 5th ed. Jaypee; 2005.
  3. Loro LL, Vintermyr OK, Johannessen AC. Cell death regulation in oral squamous cell carcinoma: methodological considerations and clinical significance. J Oral Pathol Med 2003;32:125-38.
  4. Kanduc D, Mittelman A, Serpico R, Sinigaglia E, Sinha AA, Natale C, et al. Cell death: Apoptosis versus necrosis (Review). Int J Oncol 2002;21:165-70.
  5. Gururaj N, Sivapathasundharam B. Post mortem findings in the gingiva assessed by histology and exfoliative cytology. J Oral Maxillofac Pathol 2004;8:18-21.
  6. Ritchie AC. Boyd’s Textbook of Pathology. 9th ed. Vol. 1. Philadephia/ London: Lea and Febiger; 1990.
  7. Kumar, Abbas, Fausto. Robbins and Cotrans Pathological Basis of Disease. 7th ed. Elsevier; 2004.
  8. Payne J, Busuttil JA, Smock W. Forensic Medicine Clinical and Pathological Aspects. San Francisco, London: GMM publishers; 2003.
  9. John D. Bancroft and Marilyn Gamble. Theory and Practice of Histological Techniques. 5th ed. Churchill Livingstone; 2002.