Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction


Ruchi Kishor Pawar
Chandramani B. More


Introduction: In this fast era of numerous unwanted disasters and because of the severely devastated and degenerated body remains, personal identification of unknown remains has become the most difficult and challenging task. In such instances, dental pulp plays a vital role in identification through deoxyribonucleic acid (DNA). Aim: The aim of the study is to determine sex from tooth pulp tissues by DNA analysis using polymerase chain reaction amplification method under different environmental conditions. Materials and Methods: The human extracted teeth were exposed to different conditions such as heat, soil, and open environment. The DNA was extracted from all these teeth including freshly extracted teeth, then quantified, and further amplified with male and female primers. Results: Quantity of DNA content achieved ranged from 5.21 to 62.87 ng/μl. The accuracy in determining sex from pulp DNA ranged from 92% to 100% in the study groups, except from the teeth exposed to uncontrolled heat, as the pulp tissue was burnt completely. The intergroup analysis was statistically highly significant (P < 0.001). Gender determination using the quantity of DNA was found to be nonsignificant (P > 0.05). Conclusion: The dental pulp is the reliable source for sex determination in the humid or dry environment compared to uncontrolled heat.


How to Cite
Ruchi Kishor Pawar, & Chandramani B. More. (2018). Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction. Journal of Forensic Dental Sciences, 10(2), 107–110.


  1. Verma AK, Kumar S, Rathore S, Pandey A. Role of dental expert in forensic odontology. Natl J Maxillofac Surg 2014;5:2‑5.
  2. Mayall SS, Agrawal P, Vashisht P. Dental DNA finger‑printing in identification of human remains. Ann Dent Spec 2013;1:16‑9.
  3. Taylor J. A brief history of forensic odontology and disaster victim identification practices in Australia. J Forensic Odontostomatol 2009;27:64‑74.
  4. Datta P, Sood S, Rastogi P, Bhargava K, Bhargava D, Yadav M. DNA profiling in forensic dentistry. J Indian Acad Forensic Med 2012;34:156‑9.
  5. Ata‑Ali J, Ata‑Ali F. Forensic dentistry in human identification: A review of the literature. J Clin Exp Dent 2014;6:e162‑7.
  6. Girish K, Rahman FS, Tippu SR. Dental DNA fingerprinting in identification of human remains. J Forensic Dent Sci 2010;2:63‑8.
  7. Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B, et al. Sex determination in forensic odontology: A review. J Pharm Bioallied Sci 2015;7:S398‑402.
  8. Gotherstrom A, Liden K. A modified DNA extraction method for bone and teeth. Laborativ Arkeologi 1996;9:53‑6.
  9. Naik PR, Acath DD, Sharma GH, Navalkar AR. Viability of human dental pulp in determination of sex of an individual by identifying SRY gene through DNA analysis: A single blind pilot study. JIAOMR 2012;24:133‑6.
  10. Battepati PM, Shodan M. Gender determination using primary teeth: A polymerase chain reaction (PCR) study. J Dent Oral Hyg 2013;5:77‑82.
  11. Malaver PC, Yunis JJ. Different dental tissues as source of DNA for human identification in forensic cases. Croat Med J 2003;44:306‑9.
  12. Silva RH, Quiezi R, Bertolacini CD, Carvalho SP, Gasque KC, Almeida‑e‑Silva CT, et al. Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time. RSBO 2012;9:67‑73.
  13. Vemuri S, Ramya R, Rajkumar K, Rajashree P. Influence of various environmental conditions on DNA isolation from dental pulp for sex determination using polymerase chain reaction. SRM J Res Dent Sci 2012;3:231‑6.