Role of dental pulp in age estimation:A quantitative and morphometric study


  • Anjum Baker Department of Oral Pathology, Al-Azhar Dental College, Thodupuzha, Kerala
  • K Karpagaselvi Department of Oral Pathology, Vydehi Institute of Dental Sciences and Research Centre, Bengaluru, Karnataka
  • Jayalakshmi Kumaraswamy Department of Oral Pathology, Vydehi Institute of Dental Sciences and Research Centre, Bengaluru, Karnataka
  • M Ranjini Apollo Dental Clinic, Mysore, Karnataka
  • Jabeen Gowher Armaan Dental Clinic, Bengaluru, Karnataka





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Guiglia R, Musciotto A, Compilato D, Procaccini M, Russo L, Ciavarella D, et al. Aging and oral health: Effects in hard and soft tissues. Curr Pharm Des 2010;16:619‑30.

Murray PE, Stanley HR, Matthews JB, Sloan AJ, Smith AJ. Age‑related odontometric changes of human teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:474‑82.

An G. Normal ageing of teeth. Geriatr Aging 2009;12:513‑17.

Ten Cate AR, Nanci A. Tencate’s Oral Histology Development, Structure and Function. 8th ed. India: Elsevier; 2013.

Yu C, Abbott PV. An overview of the dental pulp: Its functions and responses to injury. Aust Dent J 2007;52:S4‑16.

Avery JK, Chiego Jr., DL. Essentials of Oral Histology and Embryology: A Clinical Approach. 3rd ed. Missouri: Mosby Elsevier; 2008.

Mckenna G, Burke FM. Age‑related oral changes. Dent Update 2010;37:519‑23.

Hamet P, Tremblay J. Genes of aging. Metabolism 2003;52:5‑9.

Kumar GS, Bhasker SN, editors. Orban’s Oral Histology and Embryology. 13th ed. India: Elsevier; 2014.

Sivapathasundaram B, editor. Shafer’s Textbook of Oral Pathology. 8th ed. India: Reed Elsevier; 2015.

Hillmann G, Geurtsen W. Light‑microscopical investigation of the distribution of extracellular matrix molecules and calcifications in human dental pulps of various ages. Cell Tissue Res 1997;289:145‑54.

Suvarna SK, Layton C, Bancroft JD. Bancroft’s Theory and Practice of Histological Techniques. 7th ed. England: Churchill Livingstone Elsevier; 2013.

Culling CF, Allison RT, Barr WT. Cellular pathology technique. 4th ed. London, UK: Butterworths; 1985.

Abràmoff MD, Magalhães PJ, Ram SJ. Image processing with ImageJ. Biophotonics Int 2004;11:36‑42.

Deroulers C, Ameisen D, Badoual M, Gerin C, Granier A, Lartaud M. Analyzing huge pathology images with open source software. Diagn Pathol 2013;8:92.

Papadopulos F, Spinelli M, Valente S, Foroni L, Orrico C, Alviano F, et al. Common tasks in microscopic and ultrastructural image analysis using ImageJ. Ultrastruct Pathol 2007;31:401‑7.

Murray PE, About I, Lumley PJ, Franquin JC, Remusat M, Smith AJ. Human odontoblast cell numbers after dental injury. J Dent 2000;28:277‑85.

Vavpotic M, Turk T, Martincic DS, Balazic J. Characteristics of the number of odontoblasts in human dental pulp post‑mortem. Forensic Sci Int 2009;193:122‑6.

Tomaszewska JM, Miskowiak B, Matthews‑Brzozowska T, Wierzbicki P. Characteristics of dental pulp in human upper first premolar teeth based on immunohistochemical and morphometric examinations. Folia Histochem Cytobiol 2013;51:149‑55.

Komatsu K, Mosekilde L, Viidik A, Chiba M. Polarized light microscopic analyses of collagen fibers in the rat incisor periodontal ligament in relation to areas, regions, and ages. Anat Rec 2002;268:381‑7.

Singh M, Chaudhary AK, Pandya S, Debnath S, Singh M, Singh PA, et al. Morphometric analysis in potentially malignant head and neck lesions: Oral submucous fibrosis. Asian Pac J Cancer Prev 2010;11:257‑60.

Sanders AE. Shifting the focus of aging research into earlier decades of life. Oral Dis 2016;22:166‑8.

Gawande M, Chaudhary M, Das A. Estimation of the time of death by evaluating histological changes in the pulp. Indian J Forensic Med Toxicol 2012;6:80‑2.

Sandoval C, Nuñez M, Roa I, Sandoval C, Nuñez M, Roa I. Dental pulp fibroblast and sex determination in controlled burial conditions. Int J Morphol 2014;32:537‑41.

Viţalariu A, Căruntu ID, Bolintineanu S. Morphological changes in dental pulp after the teeth preparation procedure. Rom J Morphol Embryol 2005;46:131‑6.

Sentut T, Kirzioglu Z, Gockcimen A, Aslan H, Erdogan Y. Quantitative analysis of odontoblast cells in fluorotic and non‑fluorotic primary tooth pulp. Turkish J Med Sci 2012;42:351‑7.

Zuza EP, Carrareto AL, Lia RC, Pires JR, de Toledo BE. Histopathological features of dental pulp in teeth with different levels of chronic periodontitis severity. ISRN Dent 2012;2012:271350. [Epub 2012 Apr 10].

Caraivan O, Manolea H, Corlan Puşcu D, Fronie A, Bunget A, Mogoantă L. Microscopic aspects of pulpal changes in patients with chronic marginal periodontitis. Rom J Morphol Embryol 2012;53:725‑9.

Wan L, Lu HB, Xuan DY, Yan YX, Zhang JC. Histological changes within dental pulps in teeth with moderate‑to‑severe chronic periodontitis. Int Endod J 2015;48:95‑102.

Gates PE, Strain WD, Shore AC. Human endothelial function and microvascular ageing. Exp Physiol 2009;94:311‑6.

Catanzaro O, Dziubecki D, Lauria LC, Ceron CM, Rodriguez RR. Diabetes and its effects on dental pulp. J Oral Sci 2006;48:195‑9.

Tangjit N, Kusakabe T, Iida J. Microvasculature of dental pulp in a rat molar in an occlusal hypofunctional condition. Hokkaido J Dent Sci 2013;33:62‑71.

Popescu MR, Deva V, Dragomir LP, Searpe M, Vătu M, Stefârţă A, et al. Study on the histopathological modifications of the dental pulp in occlusal trauma. Rom J Morphol Embryol 2011;52:425‑30.

Santamaria M Jr., Milagres D, Stuani AS, Stuani MB, Ruellas AC. Initial changes in pulpal microvasculature during orthodontic tooth movement: A stereological study. Eur J Orthod 2006;28:217‑20.

Kayhan F, Küçükkeleş N, Demirel D. A histologic and histomorphometric evaluation of pulpal reactions following rapid palatal expansion. Am J Orthod Dentofacial Orthop 2000;117:465‑73.

Lovschall H, Fejerskov O, Josephsen K. Age‑related and site‑specific changes in the pulpodentinal morphology of rat molars. Arch Oral Biol 2002;47:361‑7.

Gerli R, Secciani I, Sozio F, Rossi A, Weber E, Lorenzini G. Absence of lymphatic vessels in human dental pulp: A morphological study. Eur J Oral Sci 2010;118:110‑7.

Ogrin R, Darzins P, Khalil Z. Age‑related changes in microvascular blood flow and transcutaneous oxygen tension under Basal and stimulated conditions. J Gerontol A Biol Sci Med Sci 2005;60:200‑6.

Yoshida S, Ohshima H. Distribution and organization of peripheral capillaries in dental pulp and their relationship to odontoblasts. Anat Rec 1996;245:313‑26.

Ritz‑Timme S, Cattaneo C, Collins MJ, Waite ER, Schütz HW, Kaatsch HJ, et al. Age estimation: The state of the art in relation to the specific demands of forensic practise. Int J Legal Med 2000;113:129‑36.




How to Cite

Anjum Baker, K Karpagaselvi, Jayalakshmi Kumaraswamy, M Ranjini, & Jabeen Gowher. (2019). Role of dental pulp in age estimation:A quantitative and morphometric study. Journal of Forensic Dental Sciences, 11(2), 95–102.