To Evaluate the Sensitivity of ABO Antigen Determination from Dental Pulp at Various Temperatures over a Duration of 3 Months - A Diagnostic Study

##plugins.themes.academic_pro.article.main##

Humaira Nazir
Jyothi Mahadesh
B. L. Laxmidevi
Pillai Arun Gopinathan
Ananjan Chatterjee
Pradeep L.

Abstract

Introduction: Every individual either living or dead has an unique identity as per theory of uniqueness. Medico legal examination recognizes the blood group as an unique indicator as it remains unchanged, forensic study uses teeth as the unique biological indicator, as they are the least destructible part of the body.. The present study was conducted to evaluate the efficacy in retrieving ABO blood group antigens from pulp tissue at various temperatures like freezing temperature, 100°C and 200°C with room temperature being the baseline for the study and at various time intervals (same day, 14th, 30th and 90th day) post extraction by adsorption –elution method. Materials and Methods: A diagnostic study of 80 cases was included which involved extraction procedures under local anesthesia following the aseptic protocol. Sterile cotton/gauze was compressed in the socket and later the blood collected from it served as a control group for the study. The extracted teeth were washed and kept, stored in numbered bottles for different time periods after being subjected to different degrees of temperatures i.e. at room temperature, freezing temperature, 100°C and 200°C for 60mins respectively. Results: Goodman Kruskal Gamma test was used for statistical analysis to obtain the correlation between blood groups at different temperatures. After analyzing the ABO antigen for sensitivity at various degree of temperature over different time intervals it was found that at freezing temperature and room temperature, antigens can be assessed , while at higher temperatures it is difficult to appreciate. Conclusion: Adsorption-Elution technique can be used as a reliable tool for determination of ABO blood group from the dental pulp of deceased person.

##plugins.themes.academic_pro.article.details##

How to Cite
Nazir, H. ., Mahadesh, J. ., Laxmidevi, B. L. ., Gopinathan, P. A. ., Chatterjee, A. ., & L., P. (2022). To Evaluate the Sensitivity of ABO Antigen Determination from Dental Pulp at Various Temperatures over a Duration of 3 Months - A Diagnostic Study. Journal of Forensic Dental Sciences, 12(2), 97–108. https://doi.org/10.18311/jfds/12/2/2020.612

References

  1. Stimpson PG, Mertz CA. Forensic dentistry.1st ed. London: CRC Press; 1997: Chapter 1, Scientific methods of investigations: p. 1–5.
  2. Saxana S, Sharma P, Gupta N. Experimental studies of forensic odontology to aid in the identification process. Journal of Forensic Science. 2010; 2(3):69–75. PMid: 21731343 PMCid: PMC3125956. https://doi.org/10.4103/0975-1475.81285
  3. Carl KK. Leung; Forensic Odontology. Dental Bulletin. 2008; 13(11):16–20. http://www.fmshk.org/database/articles/03db05_1.pdf?report=reader
  4. Rothwell BR. Principles of dental identification. Dent Clin North Am. 2001; 45(2):253–70.
  5. Daniels G, Bromilow I. Essential guide to blood groups.1st ed. UK: Blackwell Publication; 2000. p. 1–16.
  6. Kartika B, Elumalai M. Identify of blood group from dental pulp of deceased human. Int J Pharm Bio Sci. 2013; 4(2):1000–4.
  7. Inamdar Praphawati PI, Inamdar Praveen Kumar I, Rai K, Mirajkar AM, Venkatesh SP. Teeth- hidden treasure of blood group. Indian Journal of Forensic Medicine and 2011; 3(4):113–8. https://www.rfppl.co.in/subscription/upload_pdf/Art%203_71b.pdf
  8. Shetty M, Premalatha K. ABO blood grouping from tooth material. J Indian Acad Forensic Med. 2010; 32(4):336–8. http://medind.nic.in/jal/t10/i4/jalt10i4p336.pdf
  9. Nanci A. TenCate’s oral histology development, structure, and function; 7th ed. Haryana: Mosby Elsevier; 2010. Chapter 1, Oral Mucosa: p.1–3.
  10. Xingzhi X, Ji L, Hao F, Ming L, Zhuyao L ABO blood grouping on dental tissue. J Forensic Sci. 1993 Jul; 38(4):956–60. https://doi.org/10.1520/JFS13494J
  11. Kramer IRH. An examination of dentine for A and B blood group antigens by the mixed Agglutination Technique. Proc R Soc Med.1957 Sep; 50(9):677–8 https://doi.org/10.1177/003591575705000908
  12. Kind SS. Absorption-Elution grouping of dried blood smears. Nature. 1960; 185:397–8. PMid: 14409131. https://doi.org/10.1038/185397b0
  13. Waissbluth JG, Langman MJ. ABO blood groups, secretor status, salivary protein and serum and salivary immunoglobulin concentrations. Gut. 1971 Aug; 12(8):646–9. PMid: 18668834 PMCid: PMC1411748. https://doi.org/10.1136/gut.12.8.646
  14. Lele MV, Malvankar AG, Dange AH, Madiwale MS. Detection of ABH blood group substances in human dental pulp. Journal of the Indian Academy of Forensic Science. 1977; 16(2):1–6.
  15. Neiders ME, Standish SM. Blood grouping determination in forensic dentistry. Dent Clin North Am. 1977; 21(1):99– 111.
  16. Schemel W, Hummel K. Determination of the classical blood groups from dentine of recent, historic and prehistoric (including Paleolithic) human teeth. Forens Sci Intern.1981; 18(3):254–5. https://doi.org/10.1016/03790738(81)90042-6
  17. Haertig A ,Karinic K, Vaillant JM, Derobert L. Medico legal identification: Teeth and blood groups. Rev Stomatol Chir Maxillofac. 1980; 81(6):361–3.
  18. Bapuly AK, Das Gupta SM, Agarwal GP. Detection of ABH blood group substances from two year old dandruff samples of human origin - by Absorption-Elution Method. Journal of the Forensic Science Society of India.1984; 1:5–9.
  19. Mudd JL. A micro plate method for reverses ABO typing of bloodstains. J Forensic Sci. 1986 Apr; 31(2):418–22. https://doi.org/10.1520/JFS12271J
  20. Chattopadhyay PK, Sharma AK. Teeth as an aid to personal identification: A study for species origin and ABO blood groups. J Indian Academy of Forensic Science.1989; 28(1):25–34.
  21. Lee HC, Berka KM, Folk NL, Pagliaro EM, Carroll-Reho J, Brubaker TL, Gaensslen RE. Genetic markers in human bone: II. Studies on ABO (and IGH) Grouping. J Forensic Sci. 1991 May; 36(3):639–55. https://doi.org/10.1520/JFS13073J
  22. Smeets B, Voorde H, Hooft P. ABO blood grouping on tooth material Forensic SciInt. 1991; 50(2):277–84. https:// doi.org/10.1016/0379-0738(91)90159-G
  23. NayayamaY, Aoki Y. Distribution of ABH blood group epitopes on inner surface of dental hard tissue: Serological, Immunohistochemical and Ultrastructural study on Odontoblasts.Tohoko J Exp Med. 1998; 184:267–76. PMid: 9699242. https://doi.org/10.1620/tjem.184.267
  24. Pretty A, Sweet D. A look at forensic dentistry-Part 1: The role of teeth in the determination of human identity. Br Dent J. 2001 Apr 14; 190(7):359–66. PMid: 11338039. https://doi.org/10.1038/sj.bdj.4800972a
  25. Myers SL,Williams JM, Hodges JS. Effects of extreme heat on teeth. J Forensic Sci. 1999; 44(4):805–9. https://doi.org/10.1520/JFS14556J
  26. Nishi K, Rand S, Nakagawa T, et al. Blood typing from forensic materials-merits and demerits of detection methods utilized in our laboratories and biological significance of the antigens. Internet Journal of Forensic Medicine and Toxicology. 2005; 6(2):86–90. http://anilaggrawal.com/ij/vol_006_no_002/papers/paper001.html
  27. Ali G. Association between ABO blood groups and severity of Chronic Periodontitis. JKAU: Med.Sci. 2009; 16(3):31–41. https://www.kau.edu.sa/Files/320/Researches/57158_27403.pdf https://doi.org/10.4197/ Med.16-3.3
  28. Jadhav K, Gupta N, Mujib BR, Ahmed M, Vikram SA. Effect of acids on the teeth and its relevance on post mortem identification. J Forensic Dent Sci. 2009; 1(2):93–8. https://doi.org/10.4103/0974-2948.60381
  29. Koregol AC, Raghavendra M, Nainegali S, Kalburgi N, Varma S.ABO blood groups and Rhesus factor: An exploring link to periodontal diseases. Indian J Dent Res. 2010 Jul-Sep; 21(3):364–8. PMid: 20930346. https://doi.org/10.4103/0970-9290.70804
  30. Ballal S, David MP. Determination of ABO blood grouping from dentine and pulp. Pakistan Oral and Dental Journal. 2011; 31(1):3–6. http://podj.com.pk/archive/Jul_2011/1Podj.pdf
  31. Mondal AG, Islam MA, Rahman MM et.al. Role of blood group serology in detection, identification and investigation for criminality in Bangladesh. Dinaipur Med Col. 2011; 4(2): 83–9.
  32. Motghare P, KaleL, Bedia AS et.al. Efficacy and accuracy of ABO blood group determination from saliva. Journal of Indian Academy of Oral Medicine and Radiology. 2011; 23(3): 163–7. https://doi.org/10.5005/ jp-journals-10011-1120
  33. Aswath N, Selvamuthukumar SC, Karthika B. Role of dental pulp in identification of the deceased individual by establishing ABO blood grouping and Rhesus factor. Indian J Dent Res. 2012; 23:811–3. PMid: 23649069. https://doi.org/10.4103/0970-9290.111268
  34. Pai GP, Dayakar MM, Shaila M, Dayakar A. Correlation between ABO blood group phenotypes and periodontal disease: Prevalence in south Kanara district, Karnataka state, India. J Indian Soc Periodontol. 2012 Oct; 16(4):519–23. PMid: 23493096 PMCid: PMC3590719. https://doi.org/10.4103/0972-124X.106892
  35. RamanayakaB, Manjunath M, Joshi AA. ABO blood grouping from hard and soft tissues of teeth by modified Absorption-Elution technique. J Forensic Dent Sci. 2013 Jan; 5(1):28–34. PMid: 23960412 PMCid: PMC3746470. https://doi.org/10.4103/0975-1475.114559
  36. Vivek S, Jain J, Simon SP, Battur H, Supreetha S, Haridas R. Association of ABO blood group and RH factor with periodontal diseases in population of Virajpet, Karnataka: A cross sectional study. J Int Oral Health. 2013 Aug; 5(4):30–4.
  37. Acharya AB. An intersection of oral pathology and forensic odontology. International Journal of Oral and Maxillofacial Pathology. 2011; 2(1):12–6.
  38. Tanford C. Protein denaturation. Adv Protein Chem. 1968; 23:121–282 https://doi.org/10.1016/S0065-3233(08) 60401-5
  39. Chaplin M. Protein folding and denaturation. Current opinion in Structural Biology. 2011: 45–63. http://www1.lsbu.ac.uk/water/protein_denatured.html
  40. Lazer KL, Patapoff TW, Sharma VK. Cold denaturation of monoclonal antibodies. MAbs. 2010 Jan-Feb; 2(1):42–52. PMid: 20093856 PMCid: PMC2828577. https://doi.org/10.4161/mabs.2.1.10787
  41. Kumagai I, Tsumoto K. Antigen-antibody binding. Encyclopedia of Life Sciences. 2001. Nature Publishing Group: 1–7. http://www1.gdou.edu.cn/nxy/dwmyx/resources%5Cpdf%5C%E6%8A%97%E5%8E%9F%E6%8A%97%E4%BD%93%E7%BB%93%E5%90%88%EF%BC%88PDF%EF%BC%8C%E8%8B%B1%E6%96%87%EF%BC%89.pdf