Mohit Srivastava; Swati Tripathi; Madhusudan Astekar; Deepa Singal; Aditi Srivastava; Pallavi Vashisth

doi:10.4103/jfo.jfds_72_16

Authors

Mohit Srivastava Department of Pedodontics and Preventive Dentistry, Karnavathi School of Dentistry, Gandhinagar, Gujarat
Swati Tripathi Department of Pedodontics and Preventive Dentistry, Institute of Dental Sciences, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, 1
Madhusudan Astekar Department of Oral and Maxillofacial Pathology, Institute of Dental Sciences, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, 1
Deepa Singal Singla Dental and Eye Care Center, Moga, Punjab
Aditi Srivastava Department of Pedodontics and Preventive Dentistry, Karnavathi School of Dentistry, Gandhinagar, Gujarat
Pallavi Vashisth Department of Pedodontics and Preventive Dentistry, Institute of Dental Sciences, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, 1

DOI:

https://doi.org/10.4103/jfo.jfds_72_16

Keywords:

Amelogenin gene, mesiodens, polymerase chain reaction analysis, sex determination

Abstract

Context: The identification of sex is the first and the foremost step in forensic science. Teeth consist of enamel which is the hardest tissue available in the body, protector of DNA presents in pulp tissue at the time of exposure of tooth to adverse conditions. Teeth can be stated as a sealed box of mystery as it contains various human and bacterial DNA for molecular utilization. Aim and Objective: The aim is to determine sex from mesiodens on the basis of gene identification by the polymerase chain reaction (PCR). Settings and Design: Total number of sample was 8 human-extracted mesiodens. DNA was isolated and was subjected to PCR analysis with use of predesigned primers for amelogenin (AMEL) X and AMEL Y genes. Results: Isolation of genomic and mitochondrial DNA from mesiodens was successful in six samples (75%). In samples, quantity of DNA present was also calculated. Conclusion: Mesiodens are a good source of DNA and are a very useful tool in identification of sex using PCR analysis which was simple and effective. Hence, the procedure presented in the present study can be applied for extraction of DNA and identification of sex for forensic purpose.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J Pharm Bioallied Sci 2015;7 Suppl 2:S398-402.

Pretty IA, Sweet D. A look at forensic dentistry – Part 1: The role of teeth in the determination of human identity. Br Dent J 2001;190:359-66.

Alakoç YD, Aka PS. “Orthograde entrance technique” to recover DNA from ancient teeth preserving the physical structure. Forensic Sci Int 2009;188:96-8.

Sivagami AV, Rao AR, Varshney U. A simple and cost‑effective method for preparing DNA from the hard tooth tissue, and its use in polymerase chain reaction amplification of amelogenin gene segment for sex determination in an Indian population. Forensic Sci Int 2000;110:107-15.

Murakami H, Yamamoto Y, Yoshitome K, Ono T, Okamoto O, Shigeta Y, et al. Forensic study of sex determination using PCR on teeth samples. Acta Med Okayama 2000;54:21-32.

Cunha E, Fily ML, Clisson I, Santos AL, Silva AM. Children at the convent: Comparing historical data, morphology and DNA extracted from ancient tissues for sex diagnosis at Santa Clara-A-Velha (Coimbra, Portugal). J Archaeol Sci 2000;27:949-52.

Malaver PC, Yunis JJ. Different dental tissues as source of DNA for human identification in forensic cases. Croat Med J 2003;44:306‑9.

Qinzhang HU, Liu FE, Liu WK, Jian‑Qiang DU, Xiao‑Ming WU, et al. Sex identification of slave sacrifice victims from Qin state tombs in the spring and autumn period of china using ancient DNA. Archaeometry 2011;53:600-13.

Azlina A, Berahim Z, Sidek MD, Samsudin AR. Extraction of mitochondrial DNA from tooth dentin: Application of two techniques. Arch Orofac Sci 2011;6:9-14.

Bos KI, Schuenemann VJ, Golding GB, Burbano HA, Waglechner N, Coombes BK, et al. A draft genome of Yersinia pestis from victims of the Black Death. Nature 2011;478:506-10.

GurgulA, RadkoA, SlotaE. Characteristics of X‑ and Y‑chromosome specific regions of the amelogenin gene and a PCR‑based method for sex identification in red deer (Cervus elaphus). Mol Biol Rep 2010;37:2915-8.

Thangaraj K, Reddy AG, Singh L. Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? Int J Legal Med 2002;116:121-3.

Dayal PK. Textbook of Forensic Odontology. 1st ed.. Hyderabad: Paras Medical Publishers; 1998.

Vaòharová M, Drozdová E. Sex determination of skeletal remains of 4000-year-old children and juveniles from Hošticeza Hanou (Czech Republic) by ancient DNA analysis. Anthropol Rev 2008;71:63-70.

Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory Press; 1989.

Keohavong P, Wang CC, Cha RS, Thilly WG. Enzymatic amplification and characterization of large DNA fragments from genomic DNA. Gene 1988;71:211-6.

Pouchkarev VP, Shved EF, Novikov PI. Sex determination of forensic samples by polymerase chain reaction of the amelogenin gene and analysis by capillary electrophoresis with polymer matrix. Electrophoresis 1998;19:76-9.

Das N, Gorea RK, Gargi J, Singh JR. Sex determination from pulpal tissue. J Indian Forensic Sci 2004;26:50-4.

Galdames IS, Henríquez IR, Cantín LM. Sex chromatin in dental pulp. Performance of diagnosis test and gold standard generation. Int J Morphol 2010;28:1093-6.

Veeraraghavan G, Lingappa A, Shankara SP, Mamatha GP, Sebastian BT, MujibA. Determination of sex from tooth pulp tissue. Libyan J Med 2010;1;5

Álvarez-SandovalBA, ManzanillaLR, MontielR. Sex determination in highly fragmented human DNA by high-resolution melting (HRM) analysis. PLoS One 2014;9:e104629.

Khorate MM, Dhupar A, Ahmed J, Dinkar AD. Gender determination from pulpal tissue. J Forensic Dent Sci 2014;6:107-12.

Nayar A, Singh HP, Leekha S. Pulp tissue in sex determination: A fluorescent microscopic study. J Forensic Dent Sci 2014;6:77‑80.

Khanna KS. Efficacy of sex determination from human dental pulp tissue and its reliability as a tool in forensic dentistry. J Int Oral Health 2015;7 Suppl 2:10-6.

Praveen Kumar ST, Aswath N. DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR. J Forensic Dent Sci 2016;8:18-21.