Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study
Introduction: The present study was undertaken with the objective of ascertaining whether latent lip prints generated by persistent lipsticks and developed using lysochrome dyes have the potential of use in sex determination and personal identification. Materials and Methods: This study included a total of 100 subjects (50 males and 50 females) whose latent lip prints were obtained by applying the persistent lipstick Revlon ColorStay Overtime® manufactured by Revlon® consumer products corporation, NewYork, USA, and lifting the prints with cellophane sheets. The prints were then developed using lysochrome dyes, and all the samples were blinded and then graded based on defined patterns from the Suzuki and Tsuchihashi classification. Results: No two lip prints were found to be alike. Type I was found to be the most prevalent type. In the female population, Type I (61%) was most prevalent, followed by Type I′ (28%), Type II (9%), Type III (2%), Type IV (1%), and Type V (1%); in the male population, Type I (33%) was most prevalent, followed by Type II (23%), Type III (18%), Type IV (14%), Type I′ (10%), and Type V (3%). Two examiners were able to determine the correct sexes from the given sample sizes. Their interobserver agreement was assessed using the kappa coefficient for males (κ =0.870) and females (κ = 0.870). Their accuracy was assessed with a confidence interval (CI) of 91.48-99.38. Conclusion: Lysochrome dyes are very efficacious in developing latent lip prints. This preliminary study has conclusively proved that latent lip prints developed with lysochrome dyes hold the potential for use in sex determination and can be maintained in a digital database.
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How to Cite
Prabhath Ramakrishnan, Shraddha Bahirwani, & Smruthi Valambath. (2015). Assessment of cheiloscopy in sex determination using lysochrome - A preliminary study. Journal of Forensic Dental Sciences, 7(3), 195–200. https://doi.org/10.4103/0975-1475.172434
- Caldas IM, Magalhães T, Afonso A. Establishing identity using cheiloscopy and palatoscopy. Forensic Sci Int 2007;165:1‑9.
- Acharya AB, Sivapathasundaram B. Forensic Odontology. In: Rajendran R, Sivapathasundaram B, editor. Shafer’s Textbook of Oral Pathology. 6th ed. India: Elsevier; 2009. p. 871‑97.
- Navarro E, Castelló A, López JL, Verdú F. Criminalystic: Effectiveness of lysochromes on the developing of invisible lipstick contaminated lipmarks on human skin. A preliminary study. Forensic Sci Int 2006;158:9‑13.
- Castelló A, Alvarez M, Miguel M, Verdú F. Long‑lasting lipsticks and latent prints. Faseb J 2002;4.
- Suzuki K, Tsuchihashi Y. New attempt of personal identification by means of lip prints. J Indian Dent Assoc 1970;42:8‑9.
- Vahanwala SP, Parekh DK. Study of lip prints as an aid to forensic methodology. J Indian Dent Assoc 2000;71:269‑71.
- Vahanwala S, Nayak CD, Pagare SS. Study of lip‑prints as aid for sex determination. Medico‑legal Update 2009;5:93‑8.
- Sharma P, Saxena S, Rathod V. Cheiloscopy: The study of lip Prints in sex identification. J Forensic Dent Sci 2000;1:24‑7.
- Saraswathi TR, Mishra G, Ranganathan K. Study of lip prints. J Forensic Dent Sci 2009;1:28‑31.
- Vats Y, Dhall JK, Kapoor A. Gender variation in morphological patterns of lip prints among some north Indian populations. J Forensic Dent Sci 2012;4:19‑23.
- Kautilya DV, Bodkha P, Rajamohan N. Efficacy of cheiloscopy in determination of sex among South Indians. J Clin Diagn Res 2013;7:2193‑6.
- Navarro E, Castelló A, López‑Alfaro JA, Verdú F. More about the developing of invisible lipstick‑contaminated lipmarks on human skin: The usefulness of fluorescent dyes. J Forensic Leg Med 2007;14:340‑2.
- Kumar P, Mastan KM, Patil S. Cheiloscopy: Efficacy of Flouroscent dye over lysochrome dye in developing invisible lip prints. Int J Contemp Dent 2010;1:3‑6.
- Singh NN, Brave VR, Khanna S. Natural dyes versus lysochrome dyes in cheiloscopy: A comparative evaluation. J Forensic Dent Sci 2010;2:11‑7.
- Augustine J, Barpande SR, Tupkari JV. Cheiloscopy as an adjunct to forensic identification: A study of 600 individuals. J Forensic Odontostomatol 2008;26:44‑52.
- Gondivkar SM, Indurkar A, Degwekar S, Bhowate R. Cheiloscopy for sex determination. J Forensic Dent Sci 2009;1:56‑60.
- Patel S, Paul I, Madusudan AS, Ramesh G, Sowmya GV. A study of lip prints in relation to gender, family and blood group. IJOMP 2010;1:4‑7.
- Wadhwan V, Urs AB, Manchanda A. Sex determination using three methodologies as a tool in forensic dentistry. J Ind Dent Assoc 2011;5:77‑80.
- Singh H, Chhikara P, Singroha R. Lip prints as evidence. J Punjab Acad Forensic Med Toxicol 2011;11:23‑5.
- Malik R, Goel S. Cheiloscopy: A deterministic aid for forensic sex determination. J Indian Acad Oral Med Radiol 2011;23:17‑9.
- Sivapathasundaram B, Ajayprakash P, Sivakumar G. Lip prints (cheiloscopy). Indian J Dent Res 2001;12:234‑7.
- Prabhu RV, Dinkar AD, Prabhu VD, Rao PK. Cheiloscopy: Revisited. J Forensic Dent Sci 2012;4:47-52.