Documentation of postmortem changes in salivary gland architecture and staining characteristics

##plugins.themes.academic_pro.article.main##

Swati Agarwal
Minal Chaudhary
Madhuri Gawande
Puneet Gupta

Abstract

Context: Estimation of time passed since death continues to be a major problem for the forensic pathologist and its determination plays an important and vital role in medico-legal cases. The histological studies on various tissues after death have been mostly confined to single organ or tissue by individual workers at different atmospheric conditions. Aims: The aim of this study is to determine the best rehydrating solution for dehydrated tissues in postmortem examination. Settings and Design: This study was specific to salivary gland tissues and certain pattern of changes were determined during postmortem time intervals using hematoxylin and eosin stain and special stains like mucicarmine and alcian blue. Materials and Methods: The study was divided into two groups. (1) Group A: Normal tissue samples (twenty normal salivary gland tissue samples left without fixation for varying periods of time). (2) Group B: Control group (twenty normal salivary gland tissue samples immediately fixed in formalin). The three different rehydrating agents used in this study were glycerol, normal saline and modified Ruffer solution. Statistical Analysis Used: Not required. Results: Modified Ruffer solution is the best when compared to glycerol and normal saline for rehydration of dehydrated tissues. Conclusions: Thus in our study we conclude that the tissue which had been dehydrated at the crime scene for a fairly long period showed better rehydration with modified Ruffer solution and yield good cellular and nuclear details.

##plugins.themes.academic_pro.article.details##

How to Cite
Swati Agarwal, Minal Chaudhary, Madhuri Gawande, & Puneet Gupta. (2016). Documentation of postmortem changes in salivary gland architecture and staining characteristics. Journal of Forensic Dental Sciences, 8(2), 138–143. https://doi.org/10.4103/0975-1475.186360

References

  1. Walter JB, TalbotI C. Walter and Israel General Pathology. 7th ed. New York: Churchill Livingstone; 1996.
  2. Mohan H. Textbook of Pathology. 5th ed. New Delhi: Jaypee; 2005.
  3. Loro LL, Vintermyr OK, Johannessen AC. Cell death regulation in oral squamous cell carcinoma: Methodological considerations and clinical significance. J Oral Pathol Med 2003;32:125‑38.
  4. Kanduc D, Mittelman A, Serpico R, Sinigaglia E, Sinha AA, Natale C, et al. Cell death: Apoptosis versus necrosis (review). Int J Oncol 2002;21:165‑70.
  5. Kushwaha V, YadavM, SrivastavaAK, AgarwalA. Time since death from degenerative changes in the Kidney. J Indian Acad Forensic Med 32:37-41.
  6. Zdravković M, Kostov M, Stojanović M. Identification of postmortem autolytic changes on the kidney tissue using pas stained method. Med Biol 2006;13:181‑4.
  7. Pradeep GL, Uma K. Histological assessment of cellular changes in gingival epithelium in antemortem and post‑mortem specimens. J Forensic Dent Sci 2009;1:61-5.
  8. Schmidt CW, Nawrocki SP, Williamson MA, Marlin DC. Obtaining fingerprints from mummified fingers: A method for tissue rehydration adapted from the archeological literature. J Forensic Sci 2000;45:874‑5.