Can lip prints provide biologic evidence?

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Published Dec 1, 2016
https://doi.org/10.4103/0975-1475.195101
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Volume 8, Issue 3, September-December 2016

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Preeti Sharma
Department of Oral Pathology and Microbiology, Subharti Dental College, Meerut, Uttar Pradesh
Neeraj Sharma
KK Dental Care Centre, Meerut, Uttar Pradesh
Vijay Wadhwan
Department of Oral Pathology and Microbiology, Subharti Dental College, Meerut, Uttar Pradesh
Pooja Aggarwal
Department of Oral Pathology and Microbiology, Subharti Dental College, Meerut, Uttar Pradesh

Abstract

Background: Lip prints are unique and can be used in personal identification. Very few studies are available which establish them as biological evidence in the court of law. Thus, the objective of this study was to attempt to isolate DNA and obtain full short tandem repeat (STR) loci of the individual from the lip prints on different surfaces. Materials and Methods: Twelve lip prints were procured on different surfaces such as tissue paper, cotton cloth, ceramic tile, and glass surface. Latent lip prints were developed using fingerprint black powder. Lipstick-coated lip prints were also collected on the same supporting items. DNA was isolated, quantified, and amplified using IdentifilerTM kit to type 15 STR loci. Results: Ample quantity of DNA was extracted from all the lip print impressions and 15 loci were successfully located in seven samples. Fourteen loci were successfully typed in 3 lip impressions while 13 loci were typed in 2 samples. Conclusion: This study emphasizes the relevance of lip prints at the scene of crime. Extraction of DNA followed by typing of STR loci establishes the lip prints as biological evidence too. Tissue papers, napkins, cups, and glasses may have imprints of the suspect's lips. Thus, the full genetic profile is extremely useful for the forensic team.

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How to Cite
Preeti Sharma, Neeraj Sharma, Vijay Wadhwan, & Pooja Aggarwal. (2016). Can lip prints provide biologic evidence?. Journal of Forensic Dental Sciences, 8(3), 186–190. https://doi.org/10.4103/0975-1475.195101
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References

  1. Sivapathasundharam B, Ajayprakash P, Sivakumar G. Lip prints (Cheiloscopy). Indian J Dent Res 2001;10:234‑7.
  2. Caldas IM, Magalhães T, Afonso A. Establishing identity using cheiloscopy and palatoscopy. Forensic Sci Int 2007;165:1‑9.
  3. Sharma P, Saxena S, Rathod V. Cheiloscopy: The study of lip prints in sex identification. J Forensic Dent Sci 2009;1:24‑7.
  4. Sandhu SV, Bansal H, Monga P, Bhandari R. Study of lip print pattern in a Punjabi population. J Forensic Dent Sci 2012;4:24‑8.
  5. Kaul R, Padmashree SM, Shilpa PS, Sultana N, Bhat S. Cheiloscopic patterns in Indian population and their efficacy in sex determination: A randomized cross‑sectional study. J Forensic Dent Sci 2015;7:101‑6.
  6. Snlekha N, AnuradhaA, Srinivas GV, Devi RS. Correlation among lip print pattern, fingerprint pattern and abo blood group. J Clin Diagn Res 2014;8:49‑51.
  7. Alvarez Segui M, Miquel Feucht M, Castello Ponce A, Verdu Pascual F. Persistent lipsticks and their lip prints: New hidden evidence at the crime scene. Forensic Sci Int 2000;112:41‑7.
  8. Zamir A, Springer E, Glattstein B. Fingerprints and DNA: STR typing of DNA extracted from adhesive tape after processing for fingerprints. J Forensic Sci 2000;45:687‑8.
  9. van Oorschot RA, Jones MK. DNA fingerprints from fingerprints. Nature 1997;387:767.
  10. Schulz MM, Reichert W. Archived or directly swabbed latent fingerprints as a DNA source for STR typing. Forensic Sci Int 2002;127:128‑30.
  11. Torre C, Gino S. Epidermal cells on stubs used for detection of GSR with SEM‑EDX: Analysis of DNA polymorphisms. J Forensic Sci 1996;41:658‑9.
  12. Budowle B, Baechtel FS. Modifications to improve the effectiveness of restriction fragment length polymorphism typing. Appl Theor Electrophor 1990;1:181‑7.
  13. Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning: A Laboratory Manual 1982. 4th ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 2012.
  14. Walsh PS, Metzger DA, Higuchi R. Chele×100 as a medium for simple extraction of DNA for PCR‑based typing from forensic material. Biotechniques 1991;10:506‑13.
  15. Applied Biosystems Life Technologies Corporation. AmpFLSTR® Identifiler® PCR Amplification Kit User Guide. Minitab, Inc. Available from: https://www3.applied biosystems.com. [Last accessed on 2012 Aug].
  16. Lorente JA, Lorente M, Budowle B, Wilson MR, Villanueva E. Analysis of short tandem repeat (STR) HUMVWA in the Spanish population. Forensic Sci Int 1994;65:169‑75.
  17. Lorente M, Entrala C, Lorente JA, Alvarez JC, Villanueva E, Budowle B. Dandruff as a potential source of DNA in forensic casework. J Forensic Sci 1998;43:901‑2.
  18. Acharya AB, Sivapathasundharam B. Forensic Odontology. In: Rajendran R, Sivapathasundharam B, editors. Shafer’s Textbook of Oral Pathology. 6th ed. New Delhi: Elsevier; 2006. p. 1199‑227.
  19. Tsuchihashi Y. Studies on personal identification by means of lip prints. Forensic Sci Int 1974;3:233‑48.
  20. Castelló A, Alvarez M, Verdú F. Just lip prints? No: There could be something else. FASEB J 2004;18:615‑6.
  21. ButlerJM. Genetics and genomics of core short tandem repeat loci used in human identity testing. J Forensic Sci 2006;51:253‑65.
  22. Gill P. Role of short tandem repeat DNA in forensic casework in the UK – Past, present, and future perspectives. Biotechniques 2002;32:366‑8, 370, 372.
  23. Sharma P, Sharma N, Aggarwal P. Latent lip prints: How far a link to the suspect? European J Forensic Sci 2015;2:10‑4