Pulp tissue in sex determination: A fluorescent microscopic study

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Published Aug 1, 2014
https://doi.org/10.4103/0975-1475.132527
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Volume 6, Issue 2, May-August 2014

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Amit Nayar
Department of Oral Pathology and Microbiology, Faridkot, Punjab
Harkanwal Singh
Dasmesh Institute of Research and Dental Sciences, Faridkot, Punjab, Swami Devi Dyal Hospital and Dental College, Panchkula, Haryana
Swati Leekha
Department of Oral Pathology and Microbiology, Faridkot, Punjab

Abstract

Aims: To determine and compare the reliability of pulp tissue in determination of sex and to analyze whether caries have any effect on fluorescent body test. Materials and Methods: This study was carried on 50 maxillary and mandibular teeth (25 male teeth and 25 female teeth), which were indicated for extraction. The teeth are categorized into 5 groups, 10 each (5 from males and 5 from females) on the basis of caries progression. The pulp cells are stained with quinacrine hydrochloride and observed with fluorescent microscope for fluorescent body. Gender is determined by identification of Y chromosome fluorescence in dental pulp. Results: Fluorescent bodies were found to be more in sound teeth in males as the caries increase the mean percentage of fluorescent bodies observed decreases in males. We also observed the fluorescent spots in females, and the value of the spot increases in female as the caries progresses, thereby giving false positive results in females. Conclusion: Sex determination by fluorescent staining of the Y chromosome is a reliable technique in teeth with healthy pulps or caries with enamel or up to half way of dentin. Teeth with caries involving pulp cannot be used for sex determination.

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How to Cite
Amit Nayar, Harkanwal Singh, & Swati Leekha. (2014). Pulp tissue in sex determination: A fluorescent microscopic study. Journal of Forensic Dental Sciences, 6(2), 77–80. https://doi.org/10.4103/0975-1475.132527
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References

  1. Venkatesh G, Shrivastava R, Mutalik S. Determination of sex in South Indians and immigrant Tibetans from cephalometric analysis and discriminant functions. Forensic Sci Int 2010;197:122e1‑e6.
  2. Fixott RH. Forensic odontology. Dent Clin North Am 2001;45:217‑27.
  3. Das N, Gorea RK, Gargi J, Singh JR. Sex determination from pulpal tissue. J Indian Acad Forensic Med 2004;26:122‑5.
  4. Mubeen, Pati AR. Forensic odontology: A myriad of possibility. J Indo‑Pacific Acad Foren Odont 2010;1:11‑6.
  5. Veeraraghavan G, Lingappa A, Shankara SP, Gowda PM, Sebastian BT, Mujib A. Determination of sex from tooth pulp tissue. Libyan J Med 2010;5:5084.
  6. Caspersson T, Analysis of human interphase chromosome set by aid of DNA binding fluorescent agents. Expt Cell Res 1970;62:490‑2.
  7. Whittaker DK, Llewelyn DR. Sex determination from necrotic pulp tissue. Br Dent J 1975;139:403‑5.
  8. Seno M, Ishizu H. Sex identification of human tooth. Int J Forensic Dent 1973;1:8‑11.
  9. Koss LG, Melamed MR. Koss’ diagnostic cytology and its histopathologic bases. 5th ed. General Cytology. Lippincot Williams and Wilkin, Pennyslvania, USA, 2006. p. 62.