Assessment of morphological changes and DNA quantification: An in vitro study on acid-immersed teeth
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Abstract
Context: Acid immersion of victim′s body is one of the methods employed to subvert identification of the victim, and hence of the perpetrator. Being hardest and chemically the most stable tissue in the body, teeth can be an important forensic investigative medium in both living and nonliving populations. Teeth are also good reservoirs of both cellular and mitochondrial DNA; however, the quality and quantity of DNA obtained varies according to the environment the tooth has been subjected to. DNA extraction from acid-treated teeth has seldom been reported. Aims: The objectives of the present study were to assess the morphological changes along with DNA recovery from acid-immersed teeth. Materials and Methods: Concentrated hydrochloric acid, nitric acid, and sulfuric acid were employed for tooth decalcification. DNA was extracted on an hourly basis using phenol-chloroform method. Quantification of extracted DNA was done using a spectrophotometer. Results: Results showed that hydrochloric acid had more destructive capacity compared to other acids. Conclusion: Sufficient quantity of DNA was obtainable till the first 2 hours of acid immersion and there was an inverse proportional relation between mean absorbance ratio and quantity of obtained DNA on an hourly basis.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
K Sowmya, U Sudheendra, Samar Khan, Neelu Nagpal, & S Prathamesh. (2013). Assessment of morphological changes and DNA quantification: An in vitro study on acid-immersed teeth. Journal of Forensic Dental Sciences, 5(1), 42–46. https://doi.org/10.4103/0975-1475.114560
References
- Linjen I, Willems G. DNA research in forensic dentistry. Methods Find Exp Clin Pharmacol 2001;23:1-7.
- Mazza A, Meralatig, Savio C, Fassina G. Observation of dental structure when placed in contact with acids. J Forensic Sci 2005;50:406-10.
- John George Haigh. From Wikipedia, the free encyclopedia. http:// en.wikipedia.org/ wiki/ John_ George_Haigh/
- Leticia R, Luis JM, Ester M, Stella M. Study of short- and long- term storage of teeth and its influence on DNA. JForensic Sci 2009;54:1411-3.
- Kiran J, Nidhi G, Mujib A, Vikram SA. Effect of acid on teeth and its relevance in postmortem identification. JForensic Dent Sci 2009;1:93-8.
- Jamilly de OM, Amanda da CN, Evelyn KA, Mário HH, Regina Maria BC, Rogério Nogueira de O. Freshwater and salt-water influence in human identification by analysis of DNA: An epidemiologic and laboratory study. Braz J Oral Sci 2009;8:71-5.
- Bender K, Farfán MJ, Schneider PM. Preparation of degraded human DNA under controlled conditions. Forensic Sci Int 2004;139:135-40.
- De Leo D, Turrina S, Marigo M. Effects of individual dental factors on genomic DNA analysis. Am J Forensic Med Pathol 2000;21:411-5.
- Mukherjee KK, Biswas R. Short tandem repeat (STRs) and sex specific Amelogenin analysis of blood samples from neurosurgical female transfused patients. J Clin Forensic Med 2005;12:10-3.