Significance of Stem Cells in Forensic Dentistry


B. Karthika
Shamsul Nisa


In forensic point of view and for genetic study, biological samples collected at a crime scene serve as a significant tool, in order to resolve crimes by identifying the person. In some scenarios, individual identification gets masked by chimera persons, where the chimera person cells will have different DNA. The use of biological traces which are recorded by a person’s touch while handling items raises the chance of forensic study system. Therefore, DNA profiling can be obtained from items that were touched, which inturn becomes an useful means for forensic mode of investigation. Chimerism investigations are recognized processes to examine the condition of Hematopoietic Stem Cell Transplantation (HSCT) to analyze peripheral blood and recipient’s bone marrow samples for non-malignant and malignant hematologic diseases. In adults, ectomesenchymal cells identified in oral and maxillofacial tissues are promising for future dental stem cell therapies, because the oral tissues area rich source for stem cells. Dental stem cells have various expressive profiles and exist in specific niches. Apart from these applications, this review article highlights dental stem cells significances in forensic dental investigations.


How to Cite
Karthika, B., & Nisa, S. . (2021). Significance of Stem Cells in Forensic Dentistry. Journal of Forensic Dental Sciences, 13(1), 52–55.


  1. Goodwin W, Linacre A, Hadi S. An introduction to forensic genetics. John Wiley and Sons. 2011.
  2. Mallett X, Blythe T, Berry R. Advances in Forensic Human Identification. CRC Press. 2014. DOI:
  3. Jamal M, Chogle S, Goodis H, Karam SM. Dental stem cells and their potential role in regenerative medicine. J Med Sci, 2011; 4:53–61. https://doi. org/10.2174/1996327001104020053 DOI:
  4. Sloan AJ, Waddington RJ. Dental pulp stem cells: What, where, how? International Journal of Paediatric Dentistry. 2009; 19:61–70. PMid: 19120509. j.1365-263X.2008.00964.x DOI:
  5. 5. Potsch L, Meyer U, Rothschild S, Schneider PM, Rittner C. Application of DNA techniques for identification using human dental pulp as a source of DNA. Int J Legal Med. 1992; 105:139–43. PMid: 1419874. DOI:
  6. Sweet D, Hildebrand D. Recovery of DNA from human teeth by cryogenic grinding. J Forensic Sci. 1998; 43:1199– 202. PMid: 9846398. DOI:
  7. Sweet D, Lorente JA, Valenzuela A, Lorente M, Villanueva E. PCR-based DNA typing of saliva stains recovered from human skin. J Forensic Sci. 1997; 42:447–51. PMid: 9144934. DOI:
  8. Koh D, Ng DP, Choo SG, Ng V, Fu Q. Effect of storage conditions on the extraction of PCRquality genomic DNA from saliva. Clin Chim Acta. 2004; 343:191–4. PMid: 15115694. DOI:
  9. Khan F, Agarwal A, Agrawal S. Significance of chimerism in hematopoietic stem cell transplantation: New variations on an old theme, Bone Marrow Transplant. 2004; 34:1–12. PMid: 15156163. DOI:
  10. Thiede C. Diagnostic chimerism analysis after allogeneic stem cell transplantation: New methods and markers. Am J Pharmacogenomics. 2004; 4:177–87. PMid: 15174899. DOI:
  11. Zhou Y, Li S, Zhou J, Wang L, Song X, Lu X, Wang J, Ye Y, Ying B, Jia Y. DNA profiling in blood, buccal swabs and hair follicles of patients after allogeneic peripheral blood stem cells transplantation. Leg Med. 2011; 13(1):47–51. PMid: 21035373.
  12. Bach C, Tomova E, Goldmann K, Weisbach V, RoeslerW, Mackensen A. Winkler J, Spriewald BM. Monitoring of hematopoietic chimerism by real-time quantitative PCR of micro insertions/deletions in samples with low DNA quantities. Transfus Med Hemotherapy. 2015; 42: 38–45. PMid: 25960714 PMCid: PMC4404891. DOI:
  13. Chen DP, Tseng CP, Wang WT, Wang MC, Tsai SH, Sun CF. Real-time biallelic polymorphism-polymerase chain reaction for chimerism monitoring of hematopoietic stem cell transplantation relapsed patients. Clin Chim Acta Int J Clin Chem. 2011; 412:625–30. PMid: 21185273. DOI:
  14. Tyler J, Kumer L, Fisher C, Casey H, Shike H. Personalized chimerism test that uses selection of short tandem repeat or quantitative PCR depending on patient’s chimerism status. J Mol Diagn. 2019; 21:483–90. PMid: 30797064. DOI:
  15. Masmas TN, Madsen HO, Petersen SL, Ryder LP, Svejgaard A, Alizadeh M, Vindelov LL. Evaluation and automation of hematopoietic chimerism analysis based on real-time quantitative polymerase chain reaction. Biol Blood Marrow Transpl. 2005; 11:558–66. PMid: 15983556. DOI:
  16. Bond JW, Hammond C. The value of DNA material recovered from crime scenes. J Forensic Sci. 2008; 53:797–801. PMid: 18503525. DOI:
  17. Santurtun A, Riancho JA, Santurtún M, Richard C, Colorado MM, GarcíaUnzueta M, Zarrabeitia MT. Genetic DNA profile in urine and hair follicles from patients who have undergone allogeneic hematopoietic stem cell transplantation. Sci Justice. 2017; 57:336–40. PMid: 28889862. DOI:
  18. Li Y, Xie M, Wu J. DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation. Biomed Rep. 2014; 804–8.
  19. DOI:
  20. Zhou Y, Li S, Zhou J, Wang L, Song X, Lu X, Wang M J, Ye Y, Ying BW, Jia Y. DNA profiling in blood, buccal swabs and hair follicles of patients after allogeneic peripheral blood stem cells transplantation. Leg Med. 2011; 13:47–51. PMid: 21035373. DOI:
  21. Thiede C, Prange-Krex G, Freiberg-Richter J, Bornhauser M, Ehninger G. Buccal swabs but not mouthwash samples can be used to obtain pretransplant DNA fingerprints from recipients of allogeneic bone marrow transplants. Bone Marrow Transplant. 2000; 25(5): 575–7. PMid: 10713640. DOI:
  22. Theda C, Hwang SH, Czajko A, Loke YJ, Leong P, Craig JM. Quantitation of the cellular content of saliva and buccal swab samples. Sci Rep. 2018; 8(1):1– 8. PMid: 29720614 PMCid: PMC5932057. DOI:
  24. Tran SD, Pillemer SR, Dutra A, Barrett AJ, Brownstein MJ, Key S, Pak E, Leakan RA, Kingman A, Yamada KM, Baum BJ, Mezey E. Differentiation of human bone marrowderived cells into buccal epithelial cells in vivo: a molecular analytical study. Lancet. 2003; 361:1084–8. DOI: