Molecular insights of saliva in solving paternity dispute

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Published Apr 1, 2015
https://doi.org/10.4103/0975-1475.150325
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Volume 7, Issue 1, January-April 2015

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Madhvika Patidar
Department of Oral Pathology and Microbiology, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh
Suraksha Agrawal
Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh
Farah Parveen
Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh
Parul Khare
Department of Oral and Maxillofacial Pathology, Maharana Pratap College of Dentistry and Research Center, Gwalior, Madhya Pradesh

Abstract

Everyone is born with a unique genetic blueprint i.e. its own genome. Special locations called loci on different chromosomes display predictable inheritance patterns that could be used to determine biological relationships. These locations contain specific DNA sequences, called markers, which forensic scientists use as identifying marks for individuals. Saliva is a potentially useful source of genomic DNA for genetic studies. Paternity testing is based on the premise that we inherit half our DNA from our father and half from our mother. Therefore, persons who are biologically related must share similar DNA profile. Conversely, the absence of similarities in the DNA profiles of the child and the alleged father is used as proof that no biological relationship exists. In this paper, a female complained for being raped a year back by Mr. X and accused him of being father of her 3-months-old baby girl. DNA testing was done using saliva for the child and blood sample from the mother and the suspected father. The finding presented here allows the use of saliva as an alternative source of blood.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite
Madhvika Patidar, Suraksha Agrawal, Farah Parveen, & Parul Khare. (2015). Molecular insights of saliva in solving paternity dispute. Journal of Forensic Dental Sciences, 7(1), 76–79. https://doi.org/10.4103/0975-1475.150325
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References

  1. Henry I, Hoovers J, Barichard F, Berthéas MF, Puech A, Prieur F, et al. Pericentricintrachromosomal insertion responsible for recurrence of del (11)(p13p14) in a family. Genes Chromosomes Cancer 1993;7:57-62.
  2. Ng DP, Koh D, Choo SG, Ng V, Fu Q. Effect of storage conditions on the extraction of PCR-quality genomic DNA from saliva. Clin Chim Acta 2004;343:191-4.
  3. Ma H, Zhu H, Guan F, Cherng S. Paternity testing. JAm Sci 2006;2:76-92.
  4. Klein RD, Dykas DJ, Bale AE. Clinical testing for the nevoid basal cell carcinoma syndrome in a DNA diagnostic laboratory. Genet Med 2005;7:611-9.
  5. Khare P, Chandra S, Raj V, Agarwal S. Salivary DNA for sex determination and forensic individualization. J Forensic Med Toxicol 2012;29:73-8.
  6. Khare P, Raj V, Chandra S, Agarwal S. DNA extraction and gene amplification from saliva deposited on skin using double swab technique. Indian J Forensic Odont 2012;5:61-8.
  7. Jobling MA, Gill P. Encoded evidence: DNA in forensic analysis. Nat Rev Genet 2004;5:739-51.
  8. Adams J. Paternity testing: Blood types and DNA. Nat Educ 2008;1:146.
  9. Schanfield M. DNA parentage testing. In: Encyclopedia of Forensic Sciences. Siegel JA, Saukko PJ, Knupfer GC, editors. London: Academic Press; 2000. p. 504-15.
  10. Cifuentes LO, Martínez EH, Acuña MP, Jonquera HG. Probability of exclusion in paternity testing: Time to reassess. J Forensic Sci 2006;51:349-50.
  11. Walsh DJ, Corey AC, Cotton RW, Forman L, Herrin GL Jr, Word CJ, et al. Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva. J Forensic Sci1992;37:387-95.
  12. Sweet D, Hildebrand D. Saliva from cheese bite yields DNA profile of burglar: A case report. Int J Legal Med 1999;112: 201-3.