Age estimation by pulp-to-tooth area ratio using cone-beam computed tomography: A preliminary analysis
Background: Age estimation of living or deceased individuals is an important aspect of forensic sciences. Conventionally, pulp-to-tooth area ratio (PTR) measured from periapical radiographs have been utilized as a nondestructive method of age estimation. Cone-beam computed tomography (CBCT) is a new method to acquire three-dimensional images of the teeth in living individuals. Aims: The present study investigated age estimation based on PTR of the maxillary canines measured in three planes obtained from CBCT image data. Settings and Design: Sixty subjects aged 20–85 years were included in the study. Materials and Methods: For each tooth, mid-sagittal, mid-coronal, and three axial sections—cementoenamel junction (CEJ), one-fourth root level from CEJ, and mid-root—were assessed. PTR was calculated using AutoCAD software after outlining the pulp and tooth. Statistical Analysis Used: All statistical analyses were performed using an SPSS 17.0 software program. Results and Conclusions: Linear regression analysis showed that only PTR in axial plane at CEJ had significant age correlation (r = 0.32; P< 0.05). This is probably because of clearer demarcation of pulp and tooth outline at this level.
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How to Cite
Arpita Rai, Ashith Acharya, & Venkatesh Naikmasur. (2016). Age estimation by pulp-to-tooth area ratio using cone-beam computed tomography: A preliminary analysis. Journal of Forensic Dental Sciences, 8(3), 150–154. https://doi.org/10.4103/0975-1475.195118
- Wood RE. Forensic aspects of maxillofacial radiology. Forensic Sci Int 2006;159 Suppl 1:S47‑55.
- Gustafson G. Age determination on teeth. J Am Dent Assoc 1950;41:45-54.
- Solheim T, Vonen A. Dental age estimation, quality assurance and age estimation of asylum seekers in Norway. Forensic Sci Int 2006;159 Suppl 1:S56‑60.
- Ohtani S. Estimation of age from the teeth of unidentified corpses using the amino acid racemization method with reference to actual cases. Am J Forensic Med Pathol 1995;16:238‑42.
- Kvaal SI, Kolltveit KM, Thomsen IO, Solheim T. Age estimation of adults from dental radiographs. Forensic Sci Int 1995;74:175-85.
- Cameriere R, Ferrante L, Cingolani M. Variations in pulp/tooth area ratio as an indicator of age: A preliminary study. J Forensic Sci 2004;49:317-9.
- Cameriere R, Ferrante L, Cingolani M. Precision and reliability of pulp/tooth area ratio (RA) of second molar as indicator of adult age. J Forensic Sci 2004;49:1319-23.
- Cameriere R, Ferrante L, Belcastro MG, Bonfiglioli B, Rastelli E, Cingolani M. Age estimation by pulp/tooth ratio in canines by peri‑apical x‑rays. J Forensic Sci 2007;52:166‑70.
- Babshet M, AcharyaAB, Naikmasur VG. Age estimation in Indians from pulp/tooth area ratio of mandibular canines. Forensic Sci Int 2010;197:125.e1-4.
- Vandevoort FM, Bergmans L, Van Cleynenbreugel J, Bielen DJ, Lambrechts P, Wevers M, et al. Age calculation using x-ray microfocus computed tomographical scanning of teeth: A pilot study. J Forensic Sci 2004;49:787-90.
- Aboshi H, Takahashi T, Komuro T, Fukase Y. A method of age estimation basedon the morphometric analysis of dental pulp in mandibular first premolars by means of three-dimensional measurements taken by micro CT. Nihon Univ Dent J 2005;49:195-203.
- Someda H, Saka H, Matsunaga S, Ide Y, Nakahara K, Hirata S, et al. Age estimation based on three-dimensional measurement of mandibular central incisors in Japanese. Forensic Sci Int 2009;185:110-4.
- Aboshi H, TakahashiT, KomuroT. Age estimation using microfocus x-ray computed tomography of lower premolars. Forensic Sci Int 2010;200:35-40.
- Arai Y, Tammisalo E, Iwai K, Hashimoto K, Shinoda K. Fundamental efficiency of limited cone‑beam x‑ray CT (3DX multi image micro CT) for practical use. Dent Radiol 2000;40:145-54.
- Scarfe WC, Farman AG. Cone beam computed tomography. In: White SC, Pharoah MJ, editors. Oral Radiology: Principles and Interpretation. 6th ed., Ch. 14. St. Louis: Elsevier; 2008. p. 225-43.
- Jeevan MB, Kale AD, Angadi PV, Hallikerimath S. Age estimation by pulp/tooth area ratio in canines: Cameriere’s method assessed in an Indian sample using radiovisiography. Forensic Sci Int 2011;204:209.e1-5.
- Cameriere R, Brogi G, Ferrante L, Mirtella D, Vultaggio C, Cingolani M, et al. Reliability in age determination by pulp/ tooth ratio in upper canines in skeletal remains. J Forensic Sci 2006;51:861‑4.
- Yang F, Jacobs R, Willems G. Dental age estimation through volume matching of teeth imaged by cone-beam CT. Forensic Sci Int 2006;159 Suppl 1:S78‑83.
- Kamburoglu K, Kiliç C, Ozen T, Horasan S. Accuracy of chemically created periapical lesion measurements using limited cone beam computed tomography. Dentomaxillofac Radiol 2010;39:95-9.
- Babshet M, Acharya AB, Naikmasur VG. Age estimation from pulp/tooth area ratio (PTR) in an Indian sample: A preliminary comparison of three mandibular teeth used alone and in combination. J Forensic Leg Med 2011;18:350-4.
- Kanchan-Talreja P, Acharya AB, Naikmasur VG. An assessment of the versatility of Kvaal’s method of adult dental age estimation in Indians. Arch Oral Biol 2012;57:277-84.
- Acharya AB, Vimi S. Effectiveness of Bang and Ramm’s formulae in age assessment of Indians from dentin translucency length. Int J Legal Med 2009;123:483-8.