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ORIGINAL ARTICLE
Year : 2014  |  Volume : 6  |  Issue : 2  |  Page : 77-80

Pulp tissue in sex determination: A fluorescent microscopic study


1 Department of Oral Pathology and Microbiology, Faridkot, Punjab, India
2 Dasmesh Institute of Research and Dental Sciences, Faridkot, Punjab, Swami Devi Dyal Hospital and Dental College, Panchkula, Haryana, India

Correspondence Address:
Harkanwal Preet Singh
405 Guru Gobind Singh Nagar, Majitha Road, Amritsar 143 001
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-1475.132527

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Aims: To determine and compare the reliability of pulp tissue in determination of sex and to analyze whether caries have any effect on fluorescent body test. Materials and Methods: This study was carried on 50 maxillary and mandibular teeth (25 male teeth and 25 female teeth), which were indicated for extraction. The teeth are categorized into 5 groups, 10 each (5 from males and 5 from females) on the basis of caries progression. The pulp cells are stained with quinacrine hydrochloride and observed with fluorescent microscope for fluorescent body. Gender is determined by identification of Y chromosome fluorescence in dental pulp. Results: Fluorescent bodies were found to be more in sound teeth in males as the caries increase the mean percentage of fluorescent bodies observed decreases in males. We also observed the fluorescent spots in females, and the value of the spot increases in female as the caries progresses, thereby giving false positive results in females. Conclusion: Sex determination by fluorescent staining of the Y chromosome is a reliable technique in teeth with healthy pulps or caries with enamel or up to half way of dentin. Teeth with caries involving pulp cannot be used for sex determination.


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