Background: Blood grouping has a major role in forensic science in the field of human identification. Aim of the Study: Aim of this study was to determine ABO genotyping from pulp and dentin using PCR method. Objectives: To determine PCR based blood grouping from the DNA isolated from tooth Pulp and Dentin. Materials and Meathods: Dental pulp tissue and Dentin was collected from 20 permanent teeth and DNA extraction was carried out from pulp using (modified proteinase –K method) and from dentin using phenol/chloroform(organic method). PCR procedure was carried out and samples were subjected to agarose gel electrophoresis and blood group was determined using specific PCR primers for ABO genotyping. Results: Among the 20 samples of pulp tissue, 17 samples showed accurate results in PCR method in comparison with slide agglutination method. Among the 20 samples of dentin, blood grouping from dentin was not possible as the quantity and purity of DNA from dentin samples were not optimal. Sensitivity of 85% and specificity of 50% was noticed from the samples of pulp. Conclusion: PCR was found to be an effective method in determination of blood group from teeth. Thus our study states that isolation of DNA can be done from both pulp and dentin and the blood grouping can be done from tooth pulp by PCR method. Hence PCR method can be used for identification of individuals which adds beneficiary value to forensic dentistry.